The DNA origami method for harnessing DNA’s ability to form complex, self-assembling nanostructures as DNA origami has great potential in many areas of study. Traditional methods of origami synthesis from one pot anneals of M13, staples, and other components do not give a consistent yield from batch to batch, even when the same procedure is used. This dramatic variation of yield coupled with the absence of a reliable and rapid method for DNA origami characterization has made its transition into real world technologies difficult. Without a standardized method for quantifying the concentration and quality of DNA origami in solution, dissemination and optimization of origami-based devices will be rare. Currently, origami is most commonly characterized by gel electrophoresis—the only procedure capable of discriminating and separating differently sized DNA molecules originating from the same solution. There are drawbacks to this technique, however, including the several hours required for completion, its inability to give absolute concentrations, and the limitations on scale and yield. In this work we examine the potential for nanoparticle tracking analysis (NTA) to serve as a fast, simple and reliable method for characterizing DNA origami in solution. Furthermore, we utilize it as a tool to help us examine the aggregation phenomenon of DNA origami over time and as a function of the proportion staple strands in solution.