The Caenorhabditis elegan, a roundworm commonly used as a model organism in biology, lacks a complete analysis of its genome. Although many of its genes have been sequenced, not all of its genes are associated with a phenotype, leaving challenges in research for this organism. Some of the C. elegans’ genome is dedicated to its sensory system, and once understood may allow for insight on more complex sensory systems, such as the mammalian sensory system. An abundance of research has been conducted on C. elegans regarding their sensory system, specifically, their ability to chemosense the environment around them. In this study, we test the ability of C. elegans to chemosense diacetyl, a byproduct produced by their natural food source, to determine if certain genes play a role in chemotaxis. Repressing genes with RNA interference has enabled us to determine if certain genes have a role in C. elegans’ ability to detect diacetyl. Application of this strategy has shown several genes, previously unassociated with chemotaxis, to have an effect on the C. elegan sensory system. Furthermore, we plan to attain more information on these genes by testing C. elegan response to other volatile attractants.