The innovation engine for new materials

Sebastian Sjöström

University: 

Royal Institute of Technology

Major: 

Biochemistry

Mentor(s): 

Daniel DeMartini
William Wonderly
Eric Valois

Faculty Sponsor(s): 

Herbert Waite

Faculty Sponsor's Department: 

Cellular, Molecular and Developmental Biology

Project Title: 

BIO-INSPIRED WET ADHESION: VERIFICATION OF MUSSEL PROTEINS IN MYTILUS CALIFORNIANUS

Project Description: 

Material scientists are struggling to create adhesives that are effective in wet conditions where they conventionally perform poorly. Mytilus Californianus is a marine mussel that attaches to rocks in the intertidal zone of the ocean using proteins that adhere to a variety of substrates in dynamic conditions. In recent studies of M. californianus, transcripts of unknown proteins have been identified in the transcriptome, which may play a significant role in the mussel’s adhesive qualities. The discovery of these proteins was a result of Next-Generation Sequencing (NGS) of mRNA present in abundance in the mussel’s phenol gland. Because of the complexity of NGS, errors sometimes occur. In order to verify the discovery we used a more traditional and reliable approach - DNA cloning followed by Sanger sequencing. Result of gel electrophoresis suggest that the isolated PCR products are within the range of base pairs suggested by NGS results. Findings from these experiments provide detailed reference for comparison with results from NGS.