Recent discoveries in Gene Therapy, such as RNA interference, have led to a greater pursuit of the understanding and development of highly efficient gene carriers. These carriers can be synthetic liposomes that package nucleic acids (DNA and RNA) into complexes which in turn, deliver the nucleic acids into cells. It is important to understand the mechanism for this deliver so that further development of synthetic liposomes can be tuned for greater efficiency and lower toxicity. We used a bioluminescent assays in conjunction with DNA dot blotting that helped delineate the difference seen between delivery efficiencies of a highly fusogenic complex (using a cationic lipid DOTAP and a fusogenic lipid DOPE) and a non-fusogenic complex(DOTAP with the non-fusogenic lipid DOPC). We observed that the fusogenic complex had a 10% increased in the amount of delivered DNA and, that this seemed to correlate with a 10 fold increase in the amount of bioluminescence when compared to the non-fusogenic complex. Future research will include the separation of nuclear and cytoplasmic fractions of delivered DNA to further the understanding of the mechanism for gene delivery by these complexes.